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Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
Images
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) at 1/20000 dilution Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature.
Predicted band size: 42 kDa Observed band size: 42 kDa
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008)- ELISA Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was tested by direct ELISA. The plate was coated with 0.5 µg/mL (100 µL/well) Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was used as the secondary antibody.
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) - ELISA Cross-reactivity of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was tested by direct ELISA . The wells were coated with the indicated IgG at 0.5 µg/mL (100 µL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. A serial dilution of Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (Ab179008) was used as the secondary antibody.
Application
Application
Dilution info
ELISA
1/1000-1/10000
WB
1:5000-1:30000
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