CellGuard Certified, for cell culture, 1 M, 0.2 μm filtered
Useful PH Range
6.8-8.2
Storage Temp
Room temperature
Shipped In
Normal
Grade
CellGuard Certified, for cell culture
Product Description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application:
RNAse HEPES has been used:
To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
To recover purified ribonucleotide
As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets
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