| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| T494067-100ml | 100ml | 1 | $534.90 | |
| T494067-500ml | 500ml | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $1,868.90 |
| Specifications & Purity | Ready-to-use (RTU) |
|---|---|
| Storage Temp | Store at 2-8°C,Protected from light |
| Shipped In | Wet ice |
| Product Description | TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm. TMB EASE characteristics are: 1:1 replacement of carcinogenic 2mg OPD Aqueous based – no organic solvents (REACH Annex XVII compliant) Low lot-to-lot variation Product Characteristics TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm. TMB EASE is a ready-to-use and stable aqueous formulation. This substrate gives a slow assay reaction and is highly suitable for the direct switch from OPD (ortho-phenylenediamine dichloride) based assays. It does not contain NMP (1-methyl-2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use. Composition & Properties Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added. Working Procedure 1. The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark before use. 2. A recommended volume of 100 µl substrate solution per microtiter well is added. Time of development is typically 5-30 minutes. Keep the plate in the dark during incubation. 3. In kinetic assays, the blue color is read directly at 650 nm. For endpoint assays 100 µl (equal volumes) of 0.20 M sulphuric acid is added and the absorbance is read at 450/620 nm. The yellow solution is stable for at least one hour. Tips & Tricks • If reduced intensity is required, it is recommended to shorten the time of development or to dilute the conjugate/antibody in the immunoassay further. • Dilution of the substrate is not recommended. • In case of incidents of precipitation with very high OD-values, we can recommend the use of the TMB X-tnd (cat. no. T494100). Handling & Storage • Store solution at 2-8 °C in the dark. • Avoid exposure to light, heat and contamination with metal ions or peroxidase. • Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color. • Dispensing guidelines are available upon request. |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 F23061957 | Certificate of Analysis | Apr 27, 2023 | T494067 |
| F23061955 | Certificate of Analysis | Apr 27, 2023 | T494067 |
| F23061904 | Certificate of Analysis | Apr 27, 2023 | T494067 |
| F23061956 | Certificate of Analysis | Apr 27, 2023 | T494067 |
We have obtained your location, but we do not collect data on your location. Do we accept redirection to the corresponding region based on your location?
