C4b-binding protein is a regulatory protein of the Complement Cascade that possesses decay-accelerating activity. Circulating in plasma, it aids in the control of fluid-phase and surface-associated activation of C3 and C5 and serves as a cofactor to Factor 1 in the proteolytic degradation of C4b. There are three isoforms circulating in plasma; 7 alpha chains and 1 Beta chain, 6 alpha chains and 1 Beta chain, and 7 alpha chains without a Beta chain. The 7 alpha, 1 Beta form is the most abundant. All C4BP molecules that contain a Beta chain circulate in plasma in a high-affinity, calcium-dependent complex with vitamin K-dependent, anticoagulant Protein S. C4b-binding protein has been investigated for its role in Alzheimer's Disease, Psoriasis, as well as autoimmune diseases including Rheumatoid Arthritis, and different types of Cancer. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests. Free of Protein S.
Specifications & Purity
BioReagent, ≥95%(SDS-PAGE), Pre-lyophilization Protein Concentration
Biochemical and Physiological Mechanisms
C4BPA: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissoci
Lyophilized from 10 mM Tris pH 7.4, 100 mM NaCl, 0.05% NaN3 \r\nFree of Protein S
Concentration
Pre-lyophilization Protein Concentration
Reconstitution
After centrifugation, we suggest addition of de-ionized H2O to the original volume (See COA), followed by gentle mixingto ensure adequate homogenization. If further dilution is required, please use the lyophilization buffer.
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (2 years). Upon reconstitution, it is recommended to aliquot, store at ≤ -70℃ long term. Avoid freeze/thaw cycle.
