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Basic Description
| Synonyms | tetrasodium 4-amino-6-[(E)-2-{4'-[(E)-2-(8-amino-1-hydroxy-5,7-disulfonaphthalen-2-yl)diazen-1-yl]-3,3'-dimethyl-[1,1'-biphenyl]-4-yl}diazen-1-yl]-5-hydroxynaphthalene-1,3-disulfonate | Evans blue, sodium salt | CI Direct Blue 53 | EINECS 206-242-5 | 6,6- |
|---|---|
| Specifications & Purity | Moligand™, Biological stain |
| Biochemical and Physiological Mechanisms | Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeabi |
| Storage Temp | Desiccated |
| Shipped In | Normal |
| Grade | Biological Stain, Moligand™ |
| Note | Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details. |
Associated Targets(Human)
ALB Tchem Serum albumin (2651 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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NFKB1 Tclin Nuclear factor NF-kappa-B p105 subunit (1459 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
ATH-8 cell line (300 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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ENPP2 Tchem Autotaxin (2645 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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ENPP6 Tbio Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 (69 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
ENPP7 Tbio Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (69 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
TDP1 Tchem Tyrosyl-DNA phosphodiesterase 1 (345557 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
HSPD1 Tbio HSP60/HSP10 (366 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
TST Tchem Thiosulfate sulfurtransferase (144 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Associated Targets(non-human)
Ptpn1 Protein-tyrosine phosphatase 1B (270 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Slc17a8 Vesicular glutamate transporter 3 (89 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
PTP1 Protein-tyrosine phosphatase 1 (26 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
Luciferin 4-monooxygenase (66902 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
rep Replicase polyprotein 1ab (378 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
groL GroEL/GroES (1042 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
groEL 60 kDa chaperonin (64 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
Mechanisms of Action
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Names and Identifiers
| Pubchem Sid | 488180975 |
|---|---|
| Pubchem Sid Url | https://pubchem.ncbi.nlm.nih.gov/substance/488180975 |
| IUPAC Name | tetrasodium;4-amino-6-[[4-[4-[(8-amino-1-hydroxy-5,7-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-5-hydroxynaphthalene-1,3-disulfonate |
| INCHI | InChI=1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4 |
| InChi Key | ATNOAWAQFYGAOY-UHFFFAOYSA-J |
| Canonical SMILES | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C3)C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])O)C)N=NC5=C(C6=C(C=C5)C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+].[Na+].[Na+] |
| Isomeric SMILES | CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C3)C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])O)C)N=NC5=C(C6=C(C=C5)C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+].[Na+].[Na+] |
| WGK Germany | 3 |
| RTECS | QJ6440000 |
| Molecular Weight | 960.81 |
| Beilstein | 16(2)259 |
| Reaxy-Rn | 7110511 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=7110511&ln= |
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 C2513418 | Certificate of Analysis | Feb 27, 2025 | E104208 |
| C2513419 | Certificate of Analysis | Feb 27, 2025 | E104208 |
| C2513420 | Certificate of Analysis | Feb 27, 2025 | E104208 |
| C2513421 | Certificate of Analysis | Feb 27, 2025 | E104208 |
| J2422480 | Certificate of Analysis | Oct 10, 2024 | E104208 |
| J2422482 | Certificate of Analysis | Oct 10, 2024 | E104208 |
| J2422419 | Certificate of Analysis | Oct 10, 2024 | E104208 |
| L2206584 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| L2206710 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| L2206774 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| L2206705 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| L2206763 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| C2418046 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| L2206770 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| L2315044 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| L2206772 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| L2206688 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| H2430015 | Certificate of Analysis | Oct 22, 2022 | E104208 |
| F2214151 | Certificate of Analysis | May 25, 2022 | E104208 |
| B2317212 | Certificate of Analysis | May 25, 2022 | E104208 |
| D2419069 | Certificate of Analysis | May 25, 2022 | E104208 |
| B2317213 | Certificate of Analysis | May 25, 2022 | E104208 |
| F2214149 | Certificate of Analysis | May 25, 2022 | E104208 |
| F2214148 | Certificate of Analysis | May 25, 2022 | E104208 |
| F2214150 | Certificate of Analysis | May 25, 2022 | E104208 |
| L2315045 | Certificate of Analysis | May 25, 2022 | E104208 |
| C2418048 | Certificate of Analysis | May 25, 2022 | E104208 |
| B2317236 | Certificate of Analysis | May 25, 2022 | E104208 |
| I2227117 | Certificate of Analysis | Oct 30, 2021 | E104208 |
Show more⌵
Chemical and Physical Properties
| Solubility | Soluble in water. |
|---|---|
| Molecular Weight | 960.800 g/mol |
| XLogP3 | |
| Hydrogen Bond Donor Count | 4 |
| Hydrogen Bond Acceptor Count | 20 |
| Rotatable Bond Count | 5 |
| Exact Mass | 959.982 Da |
| Monoisotopic Mass | 959.982 Da |
| Topological Polar Surface Area | 404.000 Ų |
| Heavy Atom Count | 62 |
| Formal Charge | 0 |
| Complexity | 1790.000 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| The total count of all stereochemical bonds | 0 |
| Covalently-Bonded Unit Count | 5 |
Safety and Hazards(GHS)
| Pictogram(s) | GHS08 |
|---|---|
| Signal | Danger |
| Hazard Statements | H350:May cause cancer |
| Precautionary Statements | P280:Wear protective gloves/protective clothing/eye protection/face protection. P405:Store locked up. P501:Dispose of contents/container to ... P203:Obtain, read and follow all safety instructions before use. P318:if exposed or concerned, get medical advice. |
| WGK Germany | 3 |
| RTECS | QJ6440000 |
| Reaxy-Rn | 7110511 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=7110511&ln= |
| Merck Index | 3905 |
Specifications
| Absorbance(E1%1cm) (H2O,605.0 - 613.0 nm) | ≥550 |
|---|---|
| Loss on drying | 0-10（%） |
| Appearance（E104208) | Red to Brown to Black to Purple Powder or Crystalline Powder or Crystals or Granules |
| Infrared spectrum | Conforms to Structure |
| λ max,605.0 - 613.0 nm(H2O) | Conforms |
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