Bacterial methyl red test

This experiment is from the official website of the Fourth Military Medical University

Operation method

Bacterial methyl red test

Materials and Instruments

Escherichia coli Enterobacter aerogenes Proteus vulgaris Bacillus subtilis
NaOH solution Creatine Methyl red reagent Indole reagent Ether Bromomethylphenol violet indicator
Ultra-clean bench Constant temperature incubator Autoclave Test tubes Pipettes Duchenne cannulae Glucose peptone water medium Peptone water medium Sugar fermentation medium

Move

Test tube filled with glucose peptone water culture solution, inoculated with bacteria to be cultured and identified, after night culture, add 2 to 3 drops of methyl red indicator, pay attention to add along the wall of the tube, carefully observe the upper layer of the culture solution, if the upper layer of the culture solution becomes red, that is, a positive reaction; if it is still yellow, it is a negative reaction, respectively, with a "+" or If it is still yellow, it is a negative reaction, which is indicated by "+" or "-" respectively.

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