posted this at Jun 5, 2025 Updated at Jul 27, 2023
1
Directions per Liter:
Dissolve 53grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).
Component
g/liter
Casein Digest Peptone
5
Sodium Chloride
5
Peptic Digest of Animal
5
Beef Extract
5
Disodium Phosphate
5
Ferrous Sulfate
0.3
Bismuth Sulfite Indicator
8
Brilliant Green
0.025
Agar
20
Total: 53g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
