posted this at Jun 5, 2025 Updated at Jul 27, 2023
1
Directions per Liter:
Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
Casein Digest Peptone
5
Lactose
10
Yeast Extract
3
Brilliant Green
0.0125
Peptic Digest of Animal Tissue
5
Sodium Chloride
5
Sucrose
10
Phenol Red
0.8
Agar
20
Total: 58g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
