posted this at Jun 5, 2025 Updated at Jul 27, 2023
1
Directions per Liter:
Dissolve 202 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
Ammonium Sulfate
75
Sodium Chloride
55
Gelatin
30
Agar
15
D-Mannitol
10
Pancreatic Digest of Casein
10
Potassium Phosphate, Dibasic
5
Yeast Extract
2
Total: 202g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
