Tag Biological buffers

Articles by tag "Biological buffers"

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  1. Aladdin@Guidelines for the formulation of commonly used buffers for biological experiments In order to facilitate the experiments of scientific researchers, we have sorted out the buffer formulations commonly used in laboratory basic research in molecular biology, protein biology, microbiology and other aspects for researchers to facilitate access and use.
  2. Agrobacterium reforming process, the key technology of plant genetic engineering Plant genetic transformation technology, also called plant transgenic technology, is a key technique in modern biotechnology. It can improve plant genetic characteristics by introducing foreign genes into the plant genome. This article focuses on agrobacterium mediated method the efficiency and ...
  3. General Protocol for Western Blotting Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
  4. Western Blotting with Fluorescently Labeled Antibodies For western blotting, incubate the membrane with primary antibody diluted in 1×TBST (0.1% Tween®20) containing 5% w/v BSA or nonfat dry milk overnight at 4°C on a shaker. Please refer to the primary antibody product webpage for recommended primary antibody dilution buffer and antibody dilution.
  5. Surfactant-Free Nuclear Protein Extraction Protocol In the process of nuclear protein extraction, the use of surfactants can significantly interfere with protein labeling efficiency. This is because surfactants can alter the conformation of proteins and may interact with the target protein, affecting its biological activity and the accuracy of ...
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