Articles by tag "Buffer"

1. Buffer Reference Center
   posted this at Jun 5, 2025

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   Choose a buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature. Regulatory or purity needs for your exact application should also be considered. The following tables can help you navigate preparation of many ...
2. General Conjugation Protocols of PEG linkers——PEG Amine
   posted this at Jun 5, 2025

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   Polyethylene glycol (PEG) linker is a chemical sensing the carrier of polyethylene glycol (PEG), due to its solubility in water and non-immunogenicity. In the field of scientific research, it is widely used in chemical coupling, drug delivery, nanoparticles functionalized modifications and ...
3. General Conjugation Protocols of PEG linkers——PEG Acid
   posted this at Jun 5, 2025

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   Polyethylene glycol (PEG) linker is a chemical sensing the carrier of polyethylene glycol (PEG), due to its solubility in water and non-immunogenicity. In the field of scientific research, it is widely used in chemical coupling, drug delivery, nanoparticles functionalized modifications and ...
4. General Conjugation Protocols of PEG linkers——PEG Maleimide
   posted this at Jun 5, 2025

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   Polyethylene glycol (PEG) linker is a chemical sensing the carrier of polyethylene glycol (PEG), due to its solubility in water and non-immunogenicity. In the field of scientific research, it is widely used in chemical coupling, drug delivery, nanoparticles functionalized modifications and ...
5. General Conjugation Protocols of PEG linkers——PEG PFP Ester
   posted this at Jun 5, 2025

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   Polyethylene glycol (PEG) linker is a chemical sensing the carrier of polyethylene glycol (PEG), due to its solubility in water and non-immunogenicity. In the field of scientific research, it is widely used in chemical coupling, drug delivery, nanoparticles functionalized modifications and ...
6. Does the protein Buffer contain denaturants?
   posted this at Jun 5, 2025

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   Does the protein Buffer contain denaturants?
7. Does the protein's own charge affect the buffer?
   posted this at Jun 5, 2025

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   Does the protein's own charge affect the buffer?
8. If the concentration of the denatured sample is high, how should it be diluted?
   posted this at Jun 5, 2025

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   If the concentration of the denatured sample is high, how should it be diluted?
9. If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?
   posted this at Jun 5, 2025

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   If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?
10. What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
    posted this at Jun 5, 2025

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    What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?