The yeast is first lysed using a solution containing leaving salts to ensure complete denaturation of the macromolecules. Afterwards, ethanol is added and the D
The baculoviruses are double-stranded DNA viruses that infect mainly insects, but also in vitro insect cell lines, and are not toxic to humans or animals. Alfal
The in situ nick translation technique is also known as in situ nick translation. It was first used to study DNA replication in vitro, and has since been extend
Quantitative PCR can be used for:
(1) quantification of the amount of PCR starting template by analyzing the PCR end-product or monitoring the PCR process using
Blot hybridization is the process of binding a nucleic acid fragment to be tested to a certain solid phase support and then hybridizing it to a labeled probe pr
Agarose gel electrophoresis can be applied to (1) DNA cutting and recovery, (2) DNA isolation, (3) corroboration of whether DNA is recombinant or not, whether p
Currently, there are two main strategies for studying gene function using model mice: the gene-driven approach and the phenotype-driven approach. Gene knockout
Pyrosequencing is a luminescence-based sequencing technology for the determination of pyrophosphate (PPi). The three key points of pyrosequencing are: (1) dATPa
At present, the application of automated laser fluorescence DNA sequencing technology (also known as first generation sequencing technology) has been very commo
