Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...
When choosing a secondary antibody for western blotting, one of the main selection criteria is the species of the primary antibody that the secondary antibody binds to.
Horseradish Peroxidase (HRP) is an enzyme widely used in biochemical experiments. With its ability to catalyze the decomposition of hydrogen peroxide, HRP generates detectable products through oxidation-reduction reactions involving substrates.
