Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
For western blotting, incubate the membrane with primary antibody diluted in 1×TBST (0.1% Tween®20) containing 5% w/v BSA or nonfat dry milk overnight at 4°C on a shaker. Please refer to the primary antibody product webpage for recommended primary antibody dilution buffer and antibody dilution.
The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...
High molecular weight western blotting is a modification of the standard western blotting technique specifically designed for the detection and analysis of large molecular weight proteins.
Loading Controls are antibodies used for standardizing and quantifying the expression levels of proteins. They typically target proteins encoded by "housekeeping genes," which have relatively constant expression levels across different cell types, tissues, and physiological states, and are ...
