Tag lysate
Articles by tag "lysate"
- Immunoprecipitation (IP) lysates and reagents Immunoprecipitation is a protein purification method. An antibody for the protein of interest is incubated with a cell extract, allowing it to bind to the protein in solution. The antibody/antigen complex is then isolated from the sample using protein A/G-coupled agarose beads. This separates ...
- Lysate lysate not lysed, add ripa and shake, then lysed on ice for 20 minutes and then centrifuged and got a gooey mess? Lysate lysate not lysed, add ripa and shake, then lysed on ice for 20 minutes and then centrifuged and got a gooey mess?
- How many cells are lysed in 100μl of RIPA protein lysate? How many cells are lysed in 100μl of RIPA protein lysate?
- Can the lysate be used without sodium aluminate? Can the lysate be used without sodium aluminate?
- At what temperature is the pH 7 .4 of RIPA lysis buffer measured? At what temperature is the pH 7 .4 of RIPA lysis buffer measured?
- Does RIPA lysis buffer contain reducing agents such as DTT or mercaptoethanol? Does RIPA lysis buffer contain reducing agents such as DTT or mercaptoethanol?
- Can RIPA lysis buffer continue to be used after being stored unopened at room temperature for 24 hours? Is there a limit to the number of repeated freezing and thawing? Can RIPA lysis buffer continue to be used after being stored unopened at room temperature for 24 hours? Is there a limit to the number of repeated freezing and thawing?
- Is this lysate suitable for extracting mitochondrial proteins? Is this lysate suitable for extracting mitochondrial proteins?
- I want to obtain rare cells other than red blood cells and white blood cells in my sample and keep the original cell viability of the rare cells, is this lysate suitable? I want to obtain rare cells other than red blood cells and white blood cells in my sample and keep the original cell viability of the rare cells, is this lysate suitable?