sterile-filtered, BioReagent, ready-to-use, Suitable for Immunohistochemistry, sterile
Stability And Storage
Store at 2-8℃ for 12 months. Store at -20℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage Temp
Store at 2-8°C
Shipped In
Wet ice
Grade
BioReagent, ready-to-use, sterile, sterile-filtered, Suitable for Immunohistochemistry(IHC)
Product Description
Description This product is an optimized antibody diluent for immunostaining on the basis of traditional secondary antibody diluent, which can be used for the dilution of secondary antibody in non-fluorescent immunostaining such as immunohistochemical and immunocytochemical. Through the improvement of the detergent and the stabilizer of the second antibody, the product not only effectively reduces the non-specific binding of the second antibody, but also improves the stable preservation time of the second antibody after dilution. Procedure : 1. Reagent preparation refer to the instructions for the use of the second antibody used, as well as the quality of the primary antibody and the content of the target protein in the sample, dilute the second antibody in accordance with the appropriate proportion, such as 1:1000, 1:500 and other proportions. 2. Antibody incubation The second antibody can be incubated at the appropriate temperature after dilution of this product, and is directly used for non-fluorescent immunostaining. 3. Color reaction according to the needs of the experiment, choose the appropriate color reagent for color development. Note: 1. This product has been filtered treatment, once started, it is recommended to use within one month, if found precipitate or bacterial contamination please discard. 2. The second antibody diluted by this product can be reused 3 to 5 times within 1 to 2 weeks under appropriate storage conditions. Tips: For your own safety, please take good protection before using the reagent, such as wearing lab clothes, gloves, etc.