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o-Phenylenediamine - ≥98%, Peroxidase substrate, powder, high purity , CAS No.95-54-5

  • ≥98%
  • Peroxidase substrate
In stock
Item Number
O755484
Grouped product items
SKUSizeAvailabilityPrice Qty
O755484-50g
50g
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$208.90
O755484-100g
100g
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$378.90
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Basic Description

Specifications & Purity≥98%, Peroxidase substrate
Storage TempStore at 2-8°C
Shipped InWet ice
Product Description

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application:

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification. It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.

Names and Identifiers

IUPAC Name benzene-1,2-diamine
INCHI InChI=1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2
InChi Key GEYOCULIXLDCMW-UHFFFAOYSA-N
Canonical SMILES C1=CC=C(C(=C1)N)N
Isomeric SMILES C1=CC=C(C(=C1)N)N
WGK Germany 3
RTECS SS7875000
UN Number 1673
Packing Group III
Molecular Weight 108.14
Beilstein 606074
Reaxy-Rn 606074
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=606074&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Chemical and Physical Properties

Solubilityethanol: 50 mg/mL, clear, colorless to faintly yellow
Flash Point(°F)276.8 °F
Flash Point(°C)136°C
Boil Point(°C)256～258°C
Melt Point(°C)103-104°C
Molecular Weight108.140 g/mol
XLogP30.100
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count2
Rotatable Bond Count0
Exact Mass108.069 Da
Monoisotopic Mass108.069 Da
Topological Polar Surface Area52.000 Ų
Heavy Atom Count8
Formal Charge0
Complexity62.900
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
The total count of all stereochemical bonds0
Covalently-Bonded Unit Count1

Safety and Hazards(GHS)

Pictogram(s) GHS06,   GHS08,   GHS09
Signal Danger
Hazard Statements

H319:Causes serious eye irritation

H301:Toxic if swallowed

H341:Suspected of causing genetic defects

H351:Suspected of causing cancer

H317:May cause an allergic skin reaction

H410:Very toxic to aquatic life with long lasting effects

H312+H332:Harmful in contact with skin or if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P273:Avoid release to the environment.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P281:Use personal protective equipment as required.

P271:Use only outdoors or in a well-ventilated area.

P270:Do not eat, drink or smoke when using this product.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P272:Contaminated work clothing should not be allowed out of the workplace.

P333+P313:IF SKIN irritation or rash occurs: Get medical advice/attention.

P362+P364:Take off contaminated clothing and wash it before reuse.

P391:Collect spillage.

P330:Rinse mouth.

P203:Obtain, read and follow all safety instructions before use.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P301+P316:IF SWALLOWED: Get emergency medical help immediately.

P318:if exposed or concerned, get medical advice.

P317:Get emergency medical help.

P337+P317:If eye irritation persists: Get medical help.

WGK Germany 3
RTECS SS7875000
Reaxy-Rn 606074
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=606074&ln=
Class 6.1
Merck Index 7284

Specifications

Appearance(O755484)White to off white to tan to brown powder or flakes
Purity(GC)98-100（%）
Solubility in EtOH,Colorless to Faint Yellow Clear(50 mg/ml)Pass
Melting point98-105（℃）
NMR Spectrum 1HConforms to Structure

 Specification Sheet

Citations of This Product

How to Cite Aladdin Scientific Products?
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58. Chumeng Wang, Xinyue Yin, Lu Zhang, Nengsheng Ye, Yuhong Xiang.  (2025)  Synthesis of polyadenine-aptamer-stabilized gold nanoclusters and application to the detection of tobramycin in real samples based on their peroxidase-like activity.  FOOD CHEMISTRY,  474  (143194).  [PMID:39919421] [10.1016/j.foodchem.2025.143194]
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