Ribonuclease H (RNase H)

  • from Escherichia coli H 560 pol A1
Features and benefits
    In stock
    Item Number
    R754998
    Grouped product items
    SKUSizeAvailabilityPrice Qty
    R754998-100U
    100U
    Available within 8-12 weeks(?)
    Production requires sourcing of materials. We appreciate your patience and understanding.
    $476.90

    Basic Description

    Product NameRibonuclease H (RNase H)
    Product Description

    Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.

    Source: E. coli H560 pol A1
    Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
    Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.
    Ribonuclease H (RNase H) is a nonspecific endoribonuclease, localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.
    Application:
    Ribonuclease H (RNase H) has been used for:
    • In vivo RNA-primed initiation of DNA synthesis
    • Elimination of mRNA during second-strand cDNA synthesis
    • Site-specific cleavage of RNA
    • Detection of RNA:DNA regions in double-stranded DNA of natural origin
    • Removal of poly (A) sequences of mRNA if oligo (dT) is present
    • RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)
    Specifications & Purityfrom Escherichia coli H 560 pol A1
    Biochemical and Physiological MechanismsRibonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Usi

    Product Specifications

    Concentrationfrom Escherichia coli H 560 pol A1
    Storage TempStore at -20°C
    Shipped InIce chest + Ice pads
    Stability And StorageStore at -15–-25 °C. (unopened)

    Certificates(CoA,COO,BSE/TSE and Analysis Chart)

    C of A & Other Certificates(BSE/TSE, COO):
    Analytical Chart:

    Specifications

    Solution Calculators

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