T5 Exonuclease - EnzymoPure™, BioReagent, Exonuclease, Suitable for molecular biology, ≥95%(SDS-PAGE), ≥10 U/μL, high purity

In stock
Item Number
T750876
Grouped product items
SKUSizeAvailabilityPrice Qty
T750876-1000U
1000U
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$124.90
T750876-5000U
5000U
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$450.90
T750876-20KU
20KU
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,346.90
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Exonuclease (1)

Basic Description

SynonymsT5 Exonuclease
Specifications & PurityEnzymoPure™, BioReagent, Exonuclease, Suitable for molecular biology, ≥95%(SDS-PAGE), ≥10 U/μL
Stability And StorageStore at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
GradeBioReagent, Exonuclease, 适用于分子生物学, EnzymoPure™
Product Description

Storage buffer solution: 50mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.5, 25ºC
Product Introduction:
The T5 Exonuclease produced by Aladdin, also known as T5 nuclease, is a nuclease that degrades double stranded or single stranded DNA in the 5 '→ 3' direction. T5 Exonuclease can be digested starting from the 5 'end of single stranded or double stranded DNA, as well as from the gap or nick of linear or circular double stranded DNA. T5 Exonuclease is unable to degrade supercoiled double stranded DNA, and its activity in degrading single stranded DNA can be inhibited by reducing the Mg "2+in the reaction buffer to below 1mM. Based on these characteristics, T5 Exonuclease is commonly used for Gibson Assembly. Gibson assembly is a technique that effectively connects multiple overlapping sequence fragments under constant temperature conditions. Its basic principle can be summarized as three steps: (1) T5 nuclease digests from the 5 'end of the DNA fragment, producing complementary single stranded 3' ends (overhangs) and promoting annealing of the complementary ends; (2) DNA polymerase fills gaps in annealed fragments; (3) DNA ligase connects the assembled nick sites to obtain a complete double stranded DNA.
Purpose:
Commonly used for Gibson assembly; Degradation of linear single stranded, double stranded DNA or truncated plasmid DNA; Removing incomplete ligation products from connected circular double stranded DNA; Degradation of linear and truncated plasmid DNA to obtain high-purity supercoiled plasmid DNA; Remove the denatured plasmid DNA generated during the process of extracting plasmids using alkaline splitting method; Improve the transfection efficiency of small-scale extraction plasmid cDNA libraries.
Definition of Activity
1. The active unit (U) is the amount of enzyme required to generate 1 nmol acid soluble deoxyribonucleotide from double stranded DNA, with a total reaction volume of 50 µ l and a reaction time of 30 minutes at 37 º C.
matters needing attention:
T5 Exonuclease is a selective nuclease for DNA substrates, exhibiting varying reactivity towards different DNA substrates. Therefore, when digesting specific substrates, it is important to control the enzyme dosage and reaction time appropriately. This product is only for scientific research by professionals and should not be used for clinical diagnosis or treatment, food or medicine, or stored in ordinary residential areas. For your safety and health, please wear lab coats and disposable gloves when operating.




Names and Identifiers

PH7.5

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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