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Basic Description
| Specifications & Purity | EnzymoPure™, 50U/μl |
|---|---|
| Storage Temp | Store at -80°C |
| Shipped In | Ice chest + Ice pads |
| Grade | EnzymoPure™ |
| Product Description | Thermostable T7 RNA polymerase is a genetically engineered T7 RNA polymerase. The enzyme highly specifically recognizes the T7 promoter sequence and can be highly transcribed in vitro at 50 ℃. In the process of transcription in vitro, it can improve the capping efficiency and eliminate the production of dsRNA by-products. Product features It has high specificity for T7 promoter and is used for the synthesis of RNA (including microRNA) in vitro. Product usage 1) Synthesis of single stranded RNA; 2) Synthesis of highly specific RNA probes; 3) Synthetic siRNA precursor; 4) Making the precursor of RNA splicing reaction; 5) Cap analog was used as primer to make capped mRNA. Usage suggestions For efficient transcription of a specific region, it is suggested that the template DNA be pre cut into flat ends or 5 'protruding ends downstream of the region. Spermidine in the buffer may combine with nucleic acid to form insoluble substance. It is suggested to add template DNA finally. Storage temperature -Long term storage at 20 ℃ or -80 ℃. Quality assurance After column purification for many times, only a clear and single target band can be seen by SDS-PAGE gel detection, with a purity of 95%. No E. coli DNA residue, RNase, endonuclease and exonuclease contamination can be detected by PCR. Activity definition Under the condition of 50 ℃ and ph8.0, the amount of enzyme required to add 1nmol of [3h] GMP into the acid insoluble precipitate within 1 hour is defined as 1 active unit. |
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 B2327171 | Certificate of Analysis | Dec 22, 2023 | T489384 |
Specifications
| Titer | 45-55（IU/μl OR U/μl） |
|---|---|
| RNase contamination | No contamination |
| E. coli Genomic | No contamination |
| Endo-Nuclease contamination | No contamination |
| Exo-Nuclease contamination | No contamination |