Articles by tag "RNA Lab"

1. Natural RNA library screening and protein-specific binding RNA sequence experiments
   posted this at Jun 5, 2025

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   In cells, protein-RNA binding is involved in a variety of physiological regulation and developmental processes. Direct protein-RNA interactions occur during mRN
2. Small-scale rapid preparation of Drosophila RNA
   posted this at Jun 5, 2025

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   Guanidine hydrochloride rapidly inhibits RNAase activity while lysing cells, and this method utilizes this feature to isolate Drosophila RNA.
3. Preparation of yeast total RNA
   posted this at Jun 5, 2025

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   Good quality total RNA can be extracted from yeast cells.
4. Preparation of yeast tRNA by phenol method
   posted this at Jun 5, 2025

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   By purification on DEAE cellulose columns, small amounts of DNA, large molecular RNA, proteins, polysaccharides and other impurities were removed, and finally a
5. Preparation of fungal total RNA
   posted this at Jun 5, 2025

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   Total RNA was extracted from a variety of different fungi.
6. Yeast precursor mRNA splicing extract assay
   posted this at Jun 5, 2025

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   The splicing of eukaryotic pre-mRNA is a two-step process, in which an intron is cut from the precursor and two exons are joined together to form the mature mRN
7. Analysis of mRNA degradation experiments by cell-free extracts of higher eukaryotes
   posted this at Jun 5, 2025

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   Changes in the environment of the cell, the state of the replication cycle, and the state of differentiation can cause changes in the half-life of some mRNAs. T
8. Preparation of nuclear and cytoplasmic S100 extracts of HeLa cells for in vitro splicing reaction assay
   posted this at Jun 5, 2025

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   Cytosolic extracts can be used for splicing of precursor mRNAs. The S100 extract, although it includes many splicing factors, does not have splicing activity be
9. Purification of splicing factor SR protein assay
   posted this at Jun 5, 2025

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   Precursor mRNA ( pre-mRNA ) splicing factors The SR (serine/argininc rich) family of proteins is an important player in the assembly of splices onto precursor m
10. Preparation of antisense RNA
    posted this at Jun 5, 2025

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    RNA can also be synthesized in vitro by RNA polymerases (e.g., T3, T7, or SP6 ) using linear DNA as a template. This experiment is based on the "RNA Laboratory