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RNA related
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- AlkB (RNA/DNA demethylase, Nuclease-free)
- Species: Escherichia coli (strain K12)
- Accession #: P05050.1
- Expression system: E. coli
- Bioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
- RNase III (dsRNA-specific)
- Species: Shigella sonnei
- Accession #: B1XB41.1
- Expression system: E. coli
- Bioactivity: One unit is the amount of enzyme required to digest 1µg of dsRNA to siRNA in 20 minutes at 37ºC in a total reaction volume of 50µl.
Synonyms: Ribonuclease III (dsRNA specific) - Poly(U) Polymerase
- Species: Schizosaccharomyces pombe
- Accession #: O13833.2
- Expression system: E. coli
- Bioactivity: One unit is defined as the amount of enzyme that incorporates 1nmol of UMP into RNA in a 50μl volume in 10 minutes at 37ºC.
Synonyms: PolyU Polymerase | PUP - T4 RNA Ligase 2, truncated
- Species: Enterobacteria phage T4
- Accession #: P32277.1
- Expression system: E. coli
- Bioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.
Synonyms: T4 Rnl2, truncated | Rnl2 (1-249） | RNA ligase 2 | rnlB - T4 RNA Ligase 2, truncated KQ
- Species: Enterobacteria phage T4
- Accession #: P32277.1
- Expression system: E. coli
- Bioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.
Synonyms: T4 Rnl2tr KQ | T4 Rnl2tr R55K, K227Q
