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RNA related

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  1. AlkB (RNA/DNA demethylase, Nuclease-free)
    • Species: Escherichia coli (strain K12)
    • Accession #: P05050.1
    • Expression system: E. coli
    • Bioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
  2. T4 RNA Ligase 2, truncated
    • Species: Enterobacteria phage T4
    • Accession #: P32277.1
    • Expression system: E. coli
    • Bioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.
    Synonyms: T4 Rnl2, truncated | Rnl2 (1-249) | RNA ligase 2 | rnlB
  3. T4 RNA Ligase 2, truncated KQ
    • Species: Enterobacteria phage T4
    • Accession #: P32277.1
    • Expression system: E. coli
    • Bioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.
    Synonyms: T4 Rnl2tr KQ | T4 Rnl2tr R55K, K227Q
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