Flow cytometry enables the separation of a suspended sample of cells or particles through a thin, rapidly moving liquid stream. As the sample passes through a laser, it can detect the size, granularity, and fluorescent properties of each individual cell or particle. This technique allows for the ...
First, we should harvest our cells or tissue and prepare a single-cell suspension. We will then transfer our single-cell suspension into 96-well plates, test tubes, or polystyrene round bottom tubes, depending on the number of cells and volumes being used.
Single cells are a prerequisite for the analysis and detection of cells by flow cytometry. In the application of flow cytometry, the preparation of qualified dispersed single cells is an important part of the flow cytometry sample preparation technique. It requires both the dispersion of tissues ...
